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Files in this Data Supplement:
Movie 1. Control Ax2 cells chemotaxing towards a micropipette containing cAMP at 28°C.
Movie 2. Temperature-sensitive NSF cells attempting to chemotax towards a micropipette containing cAMP at the restrictive temperature of 28°C.
Movie 3. Temperature-sensitive NSF cells recovering at 20°C after 1 hour at the restrictive temperature of 28°C.
Movie 4. Repulsion of PTEN-GFP from the plasma membrane of a cell adjacent to a micropipette containing cAMP in temperature-sensitive NSF cells at the restrictive temperature of 28°C.
Movie 5. Recruitment of CRAC-GFP to the plasma membrane of a cell adjacent to a micropipette containing cAMP in temperature- sensitive NSF cells at the restrictive temperature of 28°C.
Movie 6. Recruitment of ABD120-GFP (a marker for F-actin) to the plasma membrane adjacent to a micropipette containing cAMP in temperature-sensitive NSF cells at the restrictive temperature of 28°C.
Movie 7. Decay of photobleached spots on the lower surface of a stationary cell expressing cAR1-GFP. Inset gives the decay kinetics.
Movie 8. Movement of photobleached mark made adjacent to a pseudopod on the lower surface of a cell moving under agarose and expressing cAR1-GFP.
Movie 9. Movement of photoactivated mark made adjacent to a pseudopod on the lower surface of a cell moving under agarose and expressing photoactivatable cAR1-GFP.
Movie 10. Movement of photobleached mark made adjacent to a pseudopod on the lower surface of a cell moving under agarose and expressing SadA-GFP.
Movie 11. Cell expressing cAR1-GFP, is moving under agarose towards cyclic-AMP. Cell surface reconstructed from confocal z-stacks taken at each time point.
Movie 12. Unconstrained cell expressing soluble GFP moving randomly. Cell surface reconstructed from confocal z-stacks taken at each time point.
Movie 13. Unconstrained cell, expressing soluble GFP moving towards a micropipette containing cAMP. Cell surface reconstructed from confocal z-stacks taken at each time point.
Movie 14. Unconstrained cell, expressing soluble GFP, which has been caused to round up by chilling. It was then warmed up and stimulated with a micropipette containing cAMP at the start of the sequence. Cell surface reconstructed from confocal z-stacks taken at each time point.
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