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Figure 1


Fig. 1. Clonal isolation and characterization of stem cells in the adult heart. (A,B) Single-cell deposition analysis was performed by the limiting dilution technique. At day 1 of the culture period, wells were inspected for the presence of single cells by phase contrast (A) and GFP fluorescence (B). (C) Colony formation from single cells in 96-well plates at 14 days of culture in serum-free medium. Three-independent colonies derived from single cells are shown. (D) FACS analysis of CSCs. Black line, control IgG; red line, corresponding antibody. Data are representative of three independent clonal CSCs. (E) RT-PCR for CSC clones. The numbers on the right indicate the number of individual colonies that expressed the corresponding genes out of the colonies examined. mES, mouse ES cells used as positive control. Data are representative of three independent clonal CSCs. Bars, 20 µm in A,B; 500 µm in C.





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