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Fig. 1. Ang II induces the tyrosine phosphorylation of 
2-adaptin. (A) VSMCs were stimulated with Ang II (1 µM) for the indicated time. Endogenous -adaptins were immunoprecipitated using the anti--adaptin antibody (AP-1/2), and the immunoprecipitates were analyzed by western blot as described in Materials and Methods. (B,C) COS-7 (B) or HEK293 cells (C) transfected with HA-AT1R and Flag-2-adaptin were stimulated with Ang II (1 µM) for the indicated time and immunoprecipitated Flag-2-adaptin was analyzed as described above. Densitometry analyses are presented as the mean ± s.e.m. of at least three independent experiments. They represent the relative amount of 2-adaptin phosphorylated as compared with non-stimulated cells after normalizing for equal amounts of 2-adaptin.
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