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Fig. 5. Recruitment of actin filaments to new cell-cell contact sites. CTRL siRNA (A-F,M-P) or WAVE2 siRNA (G-L,Q-T) treated cells were plated on collagen-coated coverslips and then incubated for 2 or 3 hours as indicated. Cells were fixed and stained for E-cadherin and actin filaments (F-actin). (C,F,I,L) Cells in B,E,H,K photographed at the apical level. (A'-L') Magnified images of the areas in the white squares in A-L, respectively. (B'',E'',H'',K'') Magnified images of B,E,H,K, respectively. Broken lines indicate the overlapping areas in H'' and K''. (M-T) vertical sections. The regions from which vertical sections were taken are indicated by white lines. Arrows indicate the locations of cell-cell contacts. Broken lines indicate the locations of coverslips. Brackets in Q and R indicate the abnormal localization of E-cadherin. Bars, 15 µm (A-L); 5 µm (A'-L'); 7.5 µm (B'',E'',H'',K''); 2.5 µm (M-T).
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