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Figure 4


Fig. 4. The tail domains of lamin Dm0 and Ce-lamin bind directly to histone H2A in vitro and the NLS motif is important for this binding. Blot overlay analysis of binding interactions between histone H2A and the lamin Dm0 tail domain; wild-type and {Delta}NLS and the Ce-lamin tail domain; wild-type and mNLS. Equal amounts of protein (5 µg) were resolved using SDS-PAGE and then transferred to a nitrocellulose membrane. Relative protein content was confirmed by Ponceau S staining of the membrane before incubation with the overlay solution (Ponceau, upper panel). Immunostaining was performed with anti-histone H2A antibody after incubation with overlay buffer containing 12 µg /ml histone H2A (ECL, lower panel). BSA was used as a negative control (not shown). Purified proteins and their corresponding overlay signals are indicated above the lanes.





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