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Figure 1


Fig. 1. RNAi loss-of-function screen to identify sorting nexins involved in retromer function. (A) Images obtained from the ArrayScan II 96 well-based, wide-field fluorescence microscopic imaging system depicting the normal steady-state enrichment of the CI-MPR in the TGN, and a redistribution of the CI-MPR into peripherally dispersed cytosolic punctae in cells suppressed for SNX1, SNX5 and SNX6. Enlarged insets, show cells scored as `normal CI-MPR staining'({circ}), cells with `dispersed CI-MPR staining' (Figure 1). (B) Data collected from >150 cells per SMARTpool, showing cells with a dispersed CI-MPR staining. Apart from the known retromer components VPS26A, VPS29 and VPS35, SNX5 and SNX6 show a high percentage of cells with altered CI-MPR staining. Transfection of cells with SNX15 or with SNX18 siRNA led to a large decrease in the number of cells due to cell death. {ddagger} indicates remaining cells that showed strong morphological changes and abnormal Golgi organization. contr. indicates cells treated with a scrambled siRNA. /. indicates untransfected control.





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