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Figure 6


Fig. 6. GFP-ECiz1 N471 resists detergent extraction after cross-linking with DTSP, but remains susceptible to salt extraction whereas GFP-C275 is resistant to both treatments. Twenty-four hours after transfection with the indicated C- or N-terminal Ciz1 fragments, cells were cross-linked with DTSP then extracted with Triton X-100 (left panels) or Triton X-100 with 0.5 M NaCl (right panels). Total nuclei were visualised after staining with Hoechst 33258 (blue) and those expressing GFP are indicated with arrows. Bar, 10 µm.





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