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Figure 4


Fig. 4. Aur-B foci colocalize more frequently with large pericentromeres and other passenger proteins in interphase nuclei. (A,B) Aur-B colocalizes frequently with pericentromeres of chromosome 1 in interphase NT2 nuclei. Aur-B (c, blue) was immunodetected with pericentromeres of chromosome 1 (b, pC1, red) in NT2 nuclei, counterstained with DAPI (a). Percentages of Aur-B positive pericentromeres in populations of NT2 nuclei exhibiting Aur-B foci are shown for chromosomes 1 and 2 (B). The number of FISH signals analyzed is indicated for each chromosome (n). (C,D) NT2 cells counterstained with DAPI (a) were detected separately with survivin (Cb, green) and INCENP (Db, green). Both passenger proteins labelled foci in a subset of interphase nuclei. (E-F) NT2 cells counterstained with DAPI (a) were co-detected with survivin and Aur-B (E) or INCENP and Aur-B (F). Merged images show that the great majority of foci detected with survivin or INCENP colocalize with Aur-B (d). Detection of survivin and INCENP in mitotic cells follow the typical passenger protein pattern (see Fig. S1 in supplementary material for survivin). Bars, 10 µm.





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