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Figure 1


Fig. 1. Pericentromeric foci of phosphorylated histone H3S10 colocalize with Aurora-B and fail to appear upon kinase inhibition. (A) Images of an interphase NT2 nucleus counterstained with DAPI (a) and labelled with the H3S10P (b, green), Aur-B (c, red) and centromeric proteins (d, blue). A merged image generated with a green mask to visualize H3S10P foci is shown (e). (B-D) A peptide competition assay was performed before immunodetection with H3S10P peptide (B), H3K9me3-S10P peptide (C) and an irrelevant H3S31P peptide (D). (E-F) Control (E) and VX-680 treated NT2 cells (F) were counterstained with DAPI (a,c) and labelled with H3S10P and Aur-B (b,c, green and red respectively). Percentages of mitotic cells (labeled M) and interphase nuclei positive for H3S10P are indicated below. DAPI-M, DAPI mask. Bars, 10 µm.





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