spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Analysis by confocal immunofluorescence microscopy of the decrease in ITPK1 expression by RNAi in MTEs. (A,B) WT MTEs grown on glass cover slips were transfected with either control, (A) non-targeting siRNA, or (B) ITPK1-specific siRNA as described in Materials and Methods. Both horizontal (X-Y) optical scanning from Z-stacks, and vertical (X-Z) scans are shown. Dotted lines show locations of accompanying perpendicular scans. ITPK1 was visualized with Alexa Fluor 488 (green) and nuclei with DAPI (blue). Bar, 10 µm. (C) Quantification of the intensity of the conjugated Alexa Fluor 488 throughout the stack volume, normalized to cell number. Data represent mean ± s.e.m. of ten independent experiments.





Right arrow Return to article