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Figure 3


Fig. 3. PLC activity in MTEs activated with UTP. WT ({blacktriangleup}) and CF (bullet) cells were labeled for 4 days with [3H]inositol. Cells were treated with vehicle (the zero time point) or UTP (for 30, 60 or 90 seconds). Individual inositol phosphate levels were determined by HPLC as described in Materials and Methods. PLC activity is computed from the increase in InsP1 + InsP2 + InsP3 + InsP4 but excluding Ins(3,4,5,6)P4 (see Carew et al., 2000 for an explanation). Data represent mean ± s.e.m. of five experiments.





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