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Figure 3


Fig. 3. Ephrin stimulation decreases the level of GTP-bound R-Ras. COS cells stably transfected with R-Ras were stimulated with ephrin-A1 Fc (A), ephrin-B1 Fc (B) or Fc as a control. GTP-bound R-Ras was isolated with GST-Raf1 RBD and detected with anti-R-Ras antibodies. The histograms show the mean levels of GTP-bound R-Ras relative to control from three experiments; the bars represent standard errors. Levels of GTP-bound R-RAS in ephrin Fc-treated cells were compared with those in Fc-treated cells by one-way ANOVA and Tukey's post-hoc test. *P<0.05; **P<0.01.





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