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Figure 5


Fig. 5. Treatment with the microtubule-stabilizing compound taxol reveals that dynamic microtubules are required for spindle integrity and proper chromosome segregation. (A) Late-anaphase cell treated with 10 µM taxol for 15 minutes. Elongated spindle microtubules are bent towards anterior of cell; lagging chromosomes remain in the spindle midzone. (B-D) Cells treated with 20 µM taxol for 1 hour. (B) Anaphase spindle morphology is normal but anaphase A chromosome segregation is delayed. (C) Anaphase spindles are broken into four half-spindle microtubule arrays. Arrows point to distal tips of broken microtubule arrays. (D) Late anaphase; spindle is bent around anterior edge of cell, with lagging chromosomes along spindle axis. Median bodies persist in mitosis and are marked with asterisks. Red, tubulin (TAT1); blue, DNA (DAPI). Bars, 2 µm.





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