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Figure 6


Fig. 6. Rab8-specific membrane turnover at the leading edge. (A) HT1080 cells expressing GFP-Rab8-67L vesicles/vacuoles (arrows) are formed at the leading edge and move toward the cell center. Dynamic tubular structures (arrowheads) are also seen to attach to and retract from the plasma membrane (supplementary material Movie 1). (B) In stably GFP-Rab8b-wt expressing NIH3T3 cells, tubular membrane structures containing GFP-Rab8b-wt are seen forming behind active ruffling lamellipodia (arrowhead), and the tubules are transported to a perinuclear region (arrows) (supplementary material Movie 2). (C) An example of membrane turnover at the leading edge of NIH3T3. GFP-Rab8b-wt positive membranes taken in from a ruffling area (arrow) fuse with a sorting vacuole (arrowhead) that turn into a tubular structure that fuses with the plasma membrane behind the lamellipodia (supplementary material Movie 3). Bars, 10 µM.





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