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Figure 3


Fig. 3. A role in transferrin recycling for Rab8. Hela cells were transfected with control (A,B,E,F) or Rab8-specific (C,D,G,H) RNAis for 48 hours. Alexa-transferrin was applied to serum starved cells for 30 minutes at 37° (Pulse) and then chased for 30 minutes at 37°C. Note that transferrin (Tfn) is localized to the perinuclear region in Rab8 expressing cells (A), but randomly in Rab8 depleted cells (C). (I) Total lysates from HeLa cells transfected with control or Rab8-specific RNAis were analyzed by western blotting by anti-actin and anti-Rab8 antibodies. Bars, 20 µM.





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