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Fig. 4. RhoA activation during myoblast differentiation and in response to cadherin signalling. (A) Western blot determination of activated (GTP-bound) RhoA and total RhoA during myoblast differentiation. (B) Western blot analysis of MHC and ß-actin in differentiating C2 myoblasts treated with the ROCK inhibitor Y-27632. (C) Western blot analysis of total and activated RhoA in lysates from C2 cells transfected with cN390
or vector alone (left panels) or with NcadFc and control secretion (right panels).