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Figure 1


Fig. 1. Increased cell density enhances myogenic differentiation of C2 cells. (A) H and E staining of C2 myoblasts seeded at the different cell densities of subconfluency (1x105 cells/60-mm-diameter plate) and confluency (4x105 cells/60-mm-diameter plate), and also at confluency in the presence of 1.75 mM EGTA; cells were stained 48 hours after the initiation of differentiation induced by transfer of cells from growth medium (GM) to differentiation medium (DM), as described in the Materials and Methods. Magnification, x50. (B) Western blot detection of MHC, phospho-p38 MAPK (pp38), total p38 MAPK and ß-actin in differentiating C2 cells at subconfluency, confluency, and confluency in the presence of 1.75 mM EGTA, sampled at the times indicated after transfer from GM to DM. (C) Northern blot of Igf2 mRNA levels in differentiating myoblasts sampled as in B; 18S rRNA ethidium bromide staining is shown as a loading control. (D) Western blot detection of N-cadherin during myogenesis sampled as in B; ß-actin is shown as a loading control.





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