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Figure 4


Fig. 4. Immunolocalization of AJ proteins in the reticular lamina. (a,c,e) Whole-mount preparations show ß-catenin (a), p120ctn (c) and ß-actin (e) localized at DC-DC junctions (asterisks and arrows) and DC-OHC junctions (arrowheads). P in (e), pillar cells. (b,d,f) ImmunoEM shows ß-catenin (b), p120ctn (d) and ß-actin (f) at DC-OHC junctions. OHCs are to the right. Black arrowheads, claudin-14-rich apical subdomain. (g) Whole-mount preparation shows E-cadherin (green) at DC-DC junctions (arrows and asterisks) and ZO-1 (red) at DC-DC (arrows) and DC-OHC junctions (arrowheads). Tissue samples are from guinea pig. Bar in g, 10 µm (a,c,e,g); 0.7 µm (b,d,f).





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