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Figure 1


Fig. 1. Morphological features of the novel DC-OHC heterotypic junction. (a) Organ of Corti schematic. Pillar Cells (stippled) separate IHC (hatching) from OHC (black asterisks). Vertical black arrows, reticular lamina. (b) DIC micrograph of the reticular lamina apical surface. Green, DC-OHC junctions; red, DC-DC junctions. P, pillar cells. (c) SEM of the apical surface and vertical cut away of the reticular lamina. (d) TEM of two DC phalanges (left and center) and an OHC (right) reveals the morphologies of DC-DC and DC-OHC junctions. Square brackets, TJ in the DC-DC AJC. (e) Freeze-etching of a DC-DC AJ (left rectangle in d) reveals fibrils across the intercellular space (white arrowheads) likely corresponding to E-cadherin cross-bridges. (f) Freeze-etching of a DC-OHC junction (right rectangle in d) reveals fibrils in a DC-OHC junction (black arrowheads) similar to those in conventional freeze-fracture replicas (Fig. 5). (g) TEM of an OHC flanked by DCs shows DC-OHC junction morphology. (h) Freeze-etching reveals a DC-OHC junction (rectangle in g). The plasma membranes (brackets) are flanked by a dense cytoplasmic protein coat (green dots) and a large actin cytoskeleton. Tissue samples from: guinea pig, (b,e-h); rat (d) and mouse (c). Black stars, DC phalanges; white arrows, DC-OHC interface; white asterisks, apices of OHCs; MT, microtubule bundle. Bar in h, 7.6 µm (b); 6.4 µm (c); 0.6 µm (d); 0.1 µm (e,f); 0.85 µm (g); 0.3 µm (h).





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