JCS03083 Supplementary Material

Files in this Data Supplement:

• Supplemental Tables - Adobe PDF

• Movie 1 -

  Movie S1 (corresponding to Fig. 7A). Example of a normal mitosis in a separase WT cell. Notice the delay between chromosome alignment and sister chromatid disjunction.

• Movie 2 -

  Movie S2 (corresponding to Fig. 7B). Example of mitosis in a separase S:A cell. Notice that chromosome alignment occurs normally. However, sister chromatid separation occurs almost immediately after the chromosomes align.

• Movie 3 -

  Movie S3 (corresponding to Fig. 7C). Example of mitosis in a separase S:A cell showing sister chromatid disjunction with unaligned chromosomes.

• Movie 4 -

  Movie S4 (corresponding to Fig. 9A). Example of a control cell expressing the GFP-Histone H2B and securin-dsRed. Notice that the securin-dsRed signal almost completely disappears prior to chromatid disjunction.

• Movie 5 -

  Movie S5 (corresponding to Fig. 9B). Example of a separase S:A cell expressing the GFP-Histone H2B and securin-dsRed. Notice that the securin-dsRed signal is not reduced prior to the premature chromatid disjunction, but does disappear upon mitotic exit.

• Movie 6 -

  Movie S6 (corresponding to Fig. 10A). Example of mitosis in a separase S:A cell expressing dsRed showing premature loss of cohesion and prolonged mitotic arrest.

• Movie 7 -

  Movie S7 (corresponding to Fig. 10B). Example of rescue phenotype, i.e. normal anaphase and mitotic exit, in a separase S:A cell expressing low levels of wild-type securin-dsRed.

• Movie 8 -

  Movie S8. Example of a cut phenotype in a separase S:A cell expressing high levels of securin-dsRed. Notice that although sister separation does not occur, securin-dsRed is degraded in late mitosis.

• Movie 9 -

  Movie S9 (corresponding to Fig. 10B). Example of a cut phenotype in a separase S:A cell expressing the securin-dsRed D-box mutant. Notice that the securin-dsRed signal dissipates at the end of mitosis because, although no longer an APC^Cdc20 substrate, the securin D-box mutant is an APC^Cdh1 substrate (Hagting et al., 2002).

• Movie 10 -

  Movie S10 (corresponding to Fig. 10B). Example of a cut phenotype in a separase S:A cell expressing the securin-dsRed D-box/KEN mutant. Notice that the securin-dsRed signal does not dissipate at the end of mitosis.