spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. ssDNA focus formation within PML-NBs is not associated with apoptotic DNA fragmentation. (A) Cells were treated with BrdU for 24 hours and subsequently irradiated with 10 J/m2 UV light or non-irradiated. Cells were then incubated in the presence or absence of caffeine for 1 or 2 hours before analysis by immunofluorescence (upper panels) or pulse field gel electrophoresis (lower panels). Bars in upper panels represent quantification of cells containing ssDNA foci. For each sample, more than 400 cells were evaluated. The average of two independent experiments ± s.d. is shown. (B) GM-847 cells were incubated in the presence of BrdU for 24 hours and then treated with the drugs indicated for 3 hours. Following treatment, cells were analyzed as in panel A by immunofluorescence labeling (upper panel) and pulse field gel electrophoresis (lower panel).





Right arrow Return to article