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Fig. 5. Co-expression of endothelial and myogenic markers in tissue sections from human fetal skeletal muscle. (A-C) Expression of M-CAM (green) with the endothelial cell marker CD31 (red). Open arrowheads indicate M-CAM+CD31- cells and filled arrowheads M-CAM+CD31+ cells. (D-F) Images from the same field demonstrating the co-expression of CD31 (red) with MyoD (green) (F is the merged image of D and E). (G) CD31 (red, open arrowhead) positive and MyoD (green, closed arrowhead) positive cells. Cells can be CD31+MyoD-. (H) CD31 (green) and Pax7 (red) are expressed by different cells and never co-localize. (I) Co-localization of M-CAM (red, open arrowhead) with N-CAM (green, filled arrowhead). Nuclei are stained in blue with DAPI. Magnification, 1000x (A,D-F,H,I); 630x (B,C,G).