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Figure 6


Fig. 6. Three-dimensional reconstruction of giant HP1-PML bodies. (A) Triple immunostaining showing PML (blue), HP1ß (green) and SP100 (red). In the middle row, the left reconstruction shows the body viewed with a slight pitch, the centre reconstruction shows the equatorial plane corresponding to the transverse sections shown in the other rows, and the right reconstruction shows the transverse section with simultaneous rendering of the three fluorescent signals. The top row shows the transverse section with the various combinations of pairs of signals. The bottom row shows the transverse section with the signals alone: left, PML sphere; centre, SP100 smaller sphere; right, HP1 small bowl. (B) Triple immunostaining showing DAXX (red), ATRX (blue) and HP1ß (green), in transverse section showing the various combinations of pairs of signals.





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