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Fig. 8. The role of PEX19-binding sites in targeting of ScPex15p. S. cerevisiae wild-type strain yHPR251 harboring an integrated copy of the synthetic peroxisomal marker PTS2-DsRed (Px) was used to express the following protein fragments fused to GFP: the C-terminal Pex15p_315-383 fragment containing both PEX19-binding sites (A); Pex15p_361-383, comprising the single C-terminal PEX19-binding site (B); the ALDP_87-164 fragment (C); Pex15p_361-383 appended to the ALDP_87-164 fragment (D); and PEX26_275-305-ALDP_87-164, the C-terminal PEX19-binding site of PEX26 fused to the same ALDP fragment (E). The transformed strains were grown on ethanol-containing plates for 2 days and inspected for GFP- and DsRed-derived fluorescence. The merged images of the two acquisitions reveal eventual colocalization of peroxisomal PTS2-DsRed with the GFP fusion proteins. DIC, differential interference contrast. Bar, 2 µm.

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