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Fig. 2. Interaction of PEX26 with PEX19 in a yeast two-hybrid assay. The indicated PEX26 fragments were fused to the Gal4p binding domain (GAL4-BD) and expressed in combination with a HsPEX19 Gal4p activation domain fusion (GAL4-AD; left panel) or the Gal4p activation domain alone (right panel) in the yeast two-hybrid strain PJ69-4a. In this assay, protein-protein interactions were assessed by the ability of the double transformants to grow on histidine-adenine double-dropout medium plates. Two independent transformants are shown for each tested interaction.