JCS02967 Supplementary Material

Files in this Data Supplement:

• Supplemental Table - Adobe PDF

• Supplemental Figure 1 - Fig. S1. Female and male gametogenesis is disrupted in Atmnd1 mutants. Mature ovule of wild type (A) and Atmnd1 (B to F) were cleared according to Motamator et al. (Motamator et al., 2000). In Arabidopsis ovules three out of the four spores degenerate immediately after meiosis. The one remaining (the functional megaspore) proceeds to three mitotic divisions, giving rise to eight cells. One of them is the egg cell, the central cell arises after fusion of two haploid nuclei, and there are two are synergid cells, whereas the three remaining, the antipodal cells, degenerate before the end of gametophyte development. Thus, in the wild type, the mature embryo sac (the female gametophyte) contains the egg cell, the central cell and two synergid cells (A). In Atmnd1 mutants 94.8% of the mature ovules contained an aborted embryo sac (B) or a single cell instead of the embryo sac (C). 2.8% of the embryo sacs were blocked at an intermediate developmental stage after one or two mitotic divisions (D and E). 2.4% of Atmnd1 mutant ovules were indistinguishable from the wild type, containing an apparently functional embryo sac (F). e, egg cell; c, central cell; s, synergid cell; n, nucleus. (G-J) Additional examples for wild-type (G) and Atmnd1 (H to J) anthers. Anthers were stained according to Alexander (Alexander, 1969). The purple-stained cytoplasm indicates viable pollen grains. 93% of Atmnd1 anthers contained no viable pollen grains (H), 6% contained one (I) and 1% contained two viable pollen grains (J). All of these pollen grains looked bigger and non-regular in shape. Bar, 20 μm (A-F); 100 μm (H-J).