JCS02973 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Subcellular localization and levels of Arm and DE-cadherin correlate with Wg expression in wing imaginal discs. A third instar wild-type wing imaginal disc was stained using triple label immunofluorescence with antibodies against Arm, DE-cadherin and Wg. The area shown is the posterior region of the wing pouch. Endogenous Wg is expressed in a stripe of approximately five cells wide along the presumptive wing margin. Staining for Arm and DE-cadherin was diffuse cytoplasmic in cells within the Wg stripe, and mostly at the plasma membrane in cells more than approximately five cell diameters away from the Wg stripe. Cells adjacent to the Wg stripe showed strongly elevated levels of Arm and DE-cadherin. Images are projections of ten serial optical sections of the apical region of wing disc cells. Anterior is to the left and dorsal up. Higher magnifications of boxed areas are shown on the right. Bar, 50 μm.

• Supplemental Figure 2 -

  Fig. S2. Overexpression of Dsh in the wing imaginal disc affects levels and subcellular localization of Arm and DE-cadherin. Dsh was overexpressed in a stripe of cells along the anterior-posterior compartment boundary of wing imaginal discs by means of the GAL4 system, using a patched-GAL4 driver. Discs were analyzed by triple label immunofluorescence staining with antibodies against Arm, DE-cadherin and Dsh. Overexpressed Dsh accumulated in large, cytoplasmic particles. Within the stripe of Dsh-overexpressing cells, Arm staining was mostly cytoplasmic and strongly reduced at the plasma membrane. In the same cells, DE-cadherin staining was increased and partially cytoplasmic. Note that the effects of Dsh overexpression were strictly cell-autonomous, since cells immediately adjacent to Dsh-overexpressing cells showed staining of Arm and DE-cadherin exclusively at the plasma membrane. Images are projections of ten serial optical sections of the apical region of wing disc cells. The large, elongated cells forming a ‘U’ around the center of the images are part of the peripodial membrane, which is closely juxtaposed to the apical surface of the wing epithelium proper. Anterior is to the left and dorsal up. Higher magnifications of boxed areas are shown on the right.