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Figure 4


Fig. 4. The expression and activity of HO-1 protein in HEK293-HO-1 cells is not altered by incubation under hypoxic conditions. (A) Western blot of cell extracts from induced (24 hours) and non-induced cells incubated under hypoxic (1% O2) and ambient (21% O2) conditions; Tet, tetracycline. (B) HO-1 activity is not affected by incubation under hypoxic or ambient conditions. (C) The concentrations of bilirubin measured in the culture medium. n=3. *P<0.05 compared with control at 21% O2; **P<0.01 compared with control at 1% O2.





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