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Fig. 5. Diffusion characteristics of eYFP-H-Ras(wt) before stimulation and 5, 10 and 15 minutes after stimulation with insulin. (A,D,G,J) Fast-diffusing fraction of molecules, , versus timelag. The solid lines show the weighted mean of the fast fraction of molecules over all timelags. (B,E,H,K) Mean square displacement of the fast-diffusing fraction, r₁², plotted versus timelag. The data were fitted according to a free diffusion model (r₁²=4D₁t_lag, solid line). (C,F,I,L) Mean square displacement of the slow-diffusing fraction, r₂², plotted versus timelag. The data in C was fitted to a free diffusion model (solid line); the data in F,I,L were fitted according to a confined diffusion model (Eqn 3, solid line). The dotted lines in the plots of the bottom row represent the offset due to the limited positional accuracy (see Materials and Methods); although the same offset is present in the plots of the middle row, the dotted lines were omitted here for clarity.

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