QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 7. Intracellular Ca²⁺-activity and annexin-binding of erythrocytes following treatment with PAF and ionomycin. Erythrocytes were loaded with Fluo-3/AM and intracellular Ca²⁺ was determined by FACS analysis, as described in Materials and Methods, after a 30-minute treatment with 3.8 µM PAF16 or 1 µM ionomycin. Additionally, annexin-binding was determined in parallel by FACS analysis. (A) Representative histograms of Ca²⁺-dependent fluorescence in FL1 of vehicle-treated erythrocytes (black line), of PAF16-treated erythrocytes (red line) or of ionomycin-treated erythrocytes (red line). Numbers give the percentage of Fluo-3 positive cells. (B) Arithmetic means ± s.e.m. (n=4) of Fluo-3-positive cells in % of the total population of vehicle-treated erythrocytes (control), PAF-treated erythrocytes (PAF16) or ionomycin-treated erythrocytes (ionomycin). * indicates significant difference from control erythrocytes (ANOVA, using Dunnett's test as post-hoc test; P $≤$ 0.05). (C) Arithmetic means ± s.e.m. (n=4) of FITC-Annexin-V-binding in % of the total population of vehicle-treated erythrocytes (control), PAF-treated erythrocytes (PAF16) or ionomycin-treated erythrocytes (ionomycin). * indicates significant difference from control erythrocytes (ANOVA, using Dunnett's test as post-hoc test; P $≤$ 0.05).

Return to article