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Fig. 2. Increase of PAF by hyperosmotic shock of erythrocytes. (A) Calibration curve of PAF using the [³H] SPA system. The normalised % of bound PAF (% B/B₀) is plotted as a function of the log₁₀ PAF concentration. Data points represent the mean of two determinations with an error less than 5% by using purified PAF as standard (20-1280 pg/tube). (B) Erythrocytes were treated with isotonic Ringer or hypertonic solution (850 mOsm) for 1 or 2 hours. Lipids were then extracted and PAF in the samples was determined with the [³H] SPA system. Arithmetic means ± s.e.m. (n=8-12) of PAF concentrations in erythrocytes exposed to isotonic (control, white bars) or hypertonic (850 mOsm, black bars) extracellular fluid are given in pg/10⁸ cells. * indicates significant difference from control (two-tailed t-test. P $≤$ 0.05). (C) Erythrocytes were treated with isotonic Ringer or hypertonic solution (850 mOsm) for 1 hour in the presence or absence of 25 µM quinacrine. Arithmetic means ± s.e.m. (n=4) of PAF concentrations in erythrocytes exposed to vehicle-containing isotonic (control) or vehicle-containing hypertonic (850 mOsm) extracellular fluid, to isotonic extracellular fluid in the presence of 25 µM quinacrine (Quin.) or to hypertonic extracellular fluid in the presence of 25 µM quinacrine (850 mOsm + Quin.). Values are given in pg/10⁸ cells; # indicates significant difference from control (ANOVA, using Dunnett's test as post hoc-test. P $≤$ 0.05).

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