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Fig. 1. Alternative splicing of CPEB1 within the RRM1. (A) Schematic representation of hCPEB1 mRNAs. Human CPEB1-long (lg) and -short (sh) mRNAs are represented with the ORF in black including the two RRM domains in grey and the zinc finger domain (Zn). The short specific 5' UTR is hatched, with the optional intron indicated with a star. The primers and the EcoNI restriction site used are indicated by arrows and scissors, respectively. The black triangle indicates the position of the alternative 15 nucleotides. (B) Comparison of CPEB1 of various species. Partial RRM1 sequences from human, murine, Xenopus and Zebrafish EST and cDNA were aligned. The GT dinucleotide corresponding to a splice donor site is highlighted in grey. The encoded amino acids are indicated below the sequence. hsCPEB1-
5 is from GenBank accession number BX327041 (nucleotides 586-630), hsCPEB1 from AF329402 (nt. 1355-1414), mmCPEB1-5 from BI144277 (nt. 395-439), mmCPEB1 from NM_007755 (nt. 1064-1123), xlCPEB from XLU14169(nt. 1114-1173) and drCPEB from AF076918 (nt. 1032-1091). (C) Position of the deletion with respect to RRM structure. 
helices and ß sheets of the RRM are represented, the black triangle indicating the position of the alternative five amino acids. (D) Differential expression of CPEB1-long and -short in tissues and cell lines. CPEB1 mRNA from indicated samples was amplified by RT-PCR using common primers rrm5 and rrm3 (upper panel), CPEB1-long specific primers lg5 and ls3 (middle panel) or CPEB1-short specific primers sh5 and ls3 (lower panel), as illustrated in A. Amplification in the absence of RNA was used as a negative control (–). A 100 bp ladder was used as molecular weight marker (M). (E) Differential expression of the 5 isoform in tissues and cell lines. RNA was amplified by RT-PCR using rrm5 and rrm3 primers and digested with EcoN1, as illustrated in A. The 
X174 HaeIII digest was used as molecular weight marker (M).
