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Fig. 1. Btn1p is the homologue of human CLN3. (A) Protein alignment between human CLN3 and its homologous proteins in mouse (Cln3) and two yeast species (Btn1), S. cerevisiae (S.cerev) and S. pombe (S.pombe). Shading indicates identical (dark) or similar (grey) residues. The position of residues mutated in S. pombe Btn1 during the course of this work that mimic those causing NCL are indicated by an asterisk (*). The likely transmembrane segments (TMS) in human CLN3 are indicated by ==. The position of TMS are based on original predictions (Janes et al., 1996), recent work (Ezaki et al., 2003; Kyttälä et al., 2003; Mao et al., 2003) and the assumption that the sequence of TMS will be conserved between mammalian and yeast species. (B) Wild-type (inset) and btn1
cells grown in YES medium at 29°C and stained with calcofluor. btn1 cells are longer at division (16±2.0 µm) than the wild-type cells from which they were derived (13±1.7 µm). Bar, 10 µm. (C) Cell-cycle length of btn1 cells (black bar) is increased compared to wild-type cells (white bar) at 29°C.
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