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Fig. 6. TIAR nuclear export is independent of CRM1 export pathway. (A) Cos cells grown on glass coverslips were incubated for 3 hours in normal medium (NT) or in medium containing actinomycin D (ActD; 5 µg/ml) or leptmomycin B (LMB; 0.2 µM) or both agents. Cells were then fixed, immunostained with anti-TIAR antibody and analyzed by confocal fluorescence microscopy as described in the Materials and Methods. Phase-contrast images are also shown (Phase). (B) Cos cells grown on coverslips were transfected with the GFP-MK2-T205,317E construct. Thirty-six hours after transfection, cells were incubated or not with leptomycin B and GFP fluorescence was analyzed as in A.
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