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Fig. 3. Subcellular localization of EGFP-TIAR hybrid proteins lacking parts of the first half of the auxiliary domain. (A) Schematic representation of TIAR protein and TIAR mutants fused to EGFP. The amino acid sequence of the first half of the auxiliary domain as well as the deletions introduced in the different mutants is specified. (B) The subcellular distribution of GFP and GFP-TIAR hybrid proteins was analyzed by epifluorescence microscopy and quantified as for Fig. 2C.





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