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Fig. 2. Effects of rapamycin on IP3- and caffeine-evoked Ca2+ increases in voltage-clamped single colonic myocytes. (A) Photolysed caged IP3 ({uparrow}) increased [Ca2+]c as indicated by F/F0. Rapamycin (10 µM, n=7) significantly reduced (P<0.05) the IP3-evoked [Ca2+]c transients. (B) Rapamycin (10 µM, n=10, P<0.01) significantly increased the Ca2+ rise evoked by caffeine (CAF, 10 mM) following activation of RyR.





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