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Fig. 6. Fluorescence activated cell-sorting analysis of Mn-SOD and frataxin expression in permeabilized and non-permeabilized Caco-2 cells. The red lines indicate the non-specific anti-mouse antibody fluorescence, while the blue and green lines indicate Mn-SOD and frataxin specific fluorescence respectively in proliferating and differentiated Caco-2 cells. (A) Non-permeabilized Caco-2 cells. Frataxin-specific cell-surface fluorescence is evident at day 13. (B) Permeabilized Caco-2 cells. (C) Percentage of surface-frataxin positive cells; analysis of one of three independent experiments.





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