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Fig. 1. The radial distribution of HSA18 and 19 in hES cells. (A) hES cell nuclei, counterstained with DAPI (blue) and hybridised with chromosome paints for HSA18 or 19. (B) Distribution of HSA18 and 19 hybridisation signals within the nucleus of H1 ES cells analysed by erosion of 2D images into five concentric shells from the edge (1) to the centre (5) of the nucleus. The mean (±s.e.m.) proportion of hybridisation signal, normalised to the amount of DAPI signal, is shown for each shell (n=50). (C) Analysis of HSA18 and 19 hybridisation signals within 3D-preserved hES cell nuclei. Graphs are the distributions of the centres of the HSA18 and 19 territories, along the fractional radius of each nucleus, along the x, y and z-axes (n=20). Bar, 5 µm.





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