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Fig. 2. PCTAIRE-1 and Sec23Ap interact directly in vitro. Purified recombinant FLAG-Sec23Ap was incubated with immobilized GST-fusion proteins as follows: GST, GST-PCTAIRE-1, GST-PCTAIRE-1(K194R) or GST-PCTAIRE-1(S153A). Beads were washed and bound protein eluted with sample buffer containing SDS, separated by SDS-PAGE and immunoblotted with an anti-FLAG antibody. Right-hand blot contains 5% of the amount of FLAG-Sec23Ap included in the binding assays (input). Position of molecular mass markers in kDa is indicated on the left.





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