(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.
Fig. 7. H2O2 promoted colocalization of myosin with the actin network in astrocytes. (A,D,G) F-actin labeled with Oregon Green. (B,E,H) Myosin Va labeled with Texas Red. (C,F,I) Colocalization of F-actin and myosin Va. (A-C) Control cells; (D-F) cells treated with H2O2 (200 µM, 2 hours); (G-I) cells treated with H2O2 (200 µM, 2 hours) and SB203580 (20 µM, 30 minutes). The images in C, F and I were obtained by suppressing all colors except yellow in Adobe Photoshop. (J) Myosin present inside the actin-enriched TNT-like connections. (K) The colocalization index of myosin with actin in astrocytes. The index is the area of coincident intensity (yellow in C,F,I) normalized by the area of noncoincident intensities (green + red – yellow). Each data point represents an average value obtained from at least 150 cells from three independent experiments. Treatment with H2O2 (200 µM, 2 hours) increased colocalization of myosin Va with actin (P<0.01), and pretreatment of cells with SB203580 (20 µM, 30 minutes) reduced this colocalization (P<0.01). Scale bars: 15 µm in A, 10 µm in J.
