QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 5. RNAi-induced knockdown of dVps16A. (A) dVps16A is expressed throughout development. Extracts for western blotting were prepared from embryos (E), first and third instar larvae (L1 and L3), pupae (P) and adult females (F) and males (M). (B) Western blots of extracts from wild-type or dVps16A RNAi knockdown larvae (0) or 1-, 2- and 3-day-old pupae (1, 2 and 3). In addition to dVps16A, Dor was drastically reduced after dVps16A knockdown, but Car, dSyntaxin7, and tubulin levels were unchanged. (C) Western blots of extracts from adult wild-type, dor¹, dor⁴ or car¹ flies did not show a reduction in dVps16A, although Dor protein levels were reduced in the dor alleles. (D) Western blots from extracts of day-four wild-type or dor⁸ third instar larvae show the loss of Dor and the reduced expression of dVps16A whereas Car, Hook, dSyn7 or tubulin were unchanged.

Return to article