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Fig. 8. Time course of MARCKS phosphorylation in the presence of DPBA. (A) Domain map for human MARCKS sequence showing the N-terminal myristoylation site and the central effector domain (ED) or PhosphoSite Domain (PSD). Based on data reviewed in Blackshear (Blackshear, 1993). (B) Typical blots for DPBA-mediated Ser159 MARCKS or Ser159/163 MARCKS phosphorylation with time after addition of the phorbol ester DPBA. (C) Average densitometry of four to six pSer159 MARCKS immunoblots. (D) Average densitometry of five to seven pSer159/163 MARCKS immunoblots.





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