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Fig. 9. Chromatin immunoprecipitation (ChIP) assay of HCT116 and Ku80+/- cells at three DNA replication origins and quantification by Real-time PCR. Real-time PCR using the LightCycler instrument was used to quantify the abundance of immunoprecipitated DNA at the origin-containing and origin-lacking regions in the three loci. (A) Quantification of lamin B2 origin-containing (LB2) or -lacking (LB2C1) regions in the three different immunoprecipitates (anti-Ku80, anti-Ku70, and NGS) from 2x107 cells. (B) Quantification of BG40.9 and BG72 DNA regions in Ku80, Ku70 and NGS immunoprecipitates. (C) Abundance of Myc11 and Myc1 DNA in Ku80, Ku70 and NGS immunoprecipitates. For all the bar graphs, the immunoprecipitating antibody is shown on the X-axis, along with the region amplified. Each error bar represents three experiments and 1 SD is indicated. An asterisk (*) represents statistically significant differences (P<0.05) between HCT116 and Ku80+/- cells in the association of the indicated protein to the specified regions.





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