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Fig. 6. D-basigin expression in the retinal photoreceptor neurons is necessary for the proper placement of their nuclei. Frozen sections through the retina (brackets in B and C); la, lamina. The normal locations of the R1-8 nuclei are marked in C. (A) Section labeled with anti-ß-gal expressed in R1-R6 nuclei. (B-G) Sections labeled with antibody to the neural nuclear protein, elav. (A) Mutant for the hypomorphic bsg allele, P1096. A few photoreceptor nuclei are displaced (arrow). (B) Mutant for bsg²⁶⁵. Nuclei are scattered throughout the retina (arrows). (C) Mutant for bsg²⁶⁵ rescued by a GMR-bsg²⁶⁵ transgene that drives expression of D-basigin 265 in all photoreceptor neurons. The nuclei of the R7 photoreceptors are just proximal to those of R1-R6 and the R8 nuclei lie near the basement membrane of the retina. (D) Mutant for bsg²⁶⁵ rescued by a GMR-mouse basigin transgene (Bsg). D-basigin and integrin interact to affect nuclear placement. (E) Male carrying a viable allele of integrin, ßPS1 (mys^b45). (F) integrin bsg²⁶⁵ double mutant male carrying mys^b45 also mutant in the eye for the P1096 bsg. Arrows indicate some of the misplaced nuclei. The number of misplaced nuclei is much greater than the sum of the two mutations independently (A and E). (G) Fly heterozygous for both the PS1 integrin allele, mew^M6 and the ßPS integrin allele mysb⁴⁵. Examples of misplaced nuclei are marked with arrows. (H) Fly mutant in the eye for Mmp2^w307, which shows no abnormality in nuclear placement. Bar, 30 µm.

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