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Fig. 3. D-basigin expressing cells spread and form lamellipodia. (A) Actin cytoskeleton of High Five cell labeled with Alexa-568 phalloidin. (B) The same cell labeled with anti-tubulin antibody and visualized with Alexa-488 conjugated secondary antibody. (C) High Five cell expressing basigin labeled with Alexa-568 phalloidin. (D) The same cell as in C labeled with antibody to tubulin. High Five cells have a spherical morphology (A and B) whereas High Five cells expressing D-basigin form lamellipodia (C and D). Bar, 40 µm.