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Fig. 7. CaKar9p is delocalized in the Cabni1{Delta} mutant. One copy of the CaKAR9 gene in both the wild-type (CaBNI1, strain WYL17) and Cabni1{Delta} (strain WYL21) strains was tagged with GFP under the control of the MET3 promoter. (A) Exponential phase yeast cells. (B) Hyphal cells. (C) The cortical localization of CaKar9p depends on actin and microtubules. Random yeast cells and 2-hour hyphal cells of strain WYL17 were treated with either 200 µM LAT-A or 40 µM nocodazole (NOC) for 30 min. Bars, 5 µm.





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