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Fig. 1. The ER fails to distribute to the periphery of daughter cells in the absence of Ice2p. Wild-type and ice2 mutant cells expressing the ER markers Hmg1p-GFP (A), or Sec61p-GFP (B) were grown at 30°C to early log phase in minimal medium with the appropriate amino acids. Asterisks indicate small buds in ice2 cells without cortical ER; arrows and arrowheads point to buds with a concentration of ER at the tip. The ER distribution in small buds with a volume of 4-13% of the mother cell was analysed using fluorescence microscopy.





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