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Fig. 4. The rate of cell migration is inversely proportional to the distance from the wound edge, and cells move along related, though still partly independent, paths toward the wound area. The row number refers to rough distance from the wound margin in terms of cell rows, with row 1 being cells at the margin, and rows 2-5 and 10 being submarginal cells progressively further from the edge of the cell sheet. Centroids from individual cells in the wounded MDCK cell monolayer were tracked for 12 hours after wounding. The initial width of the rectangular wounds was 
700 µm. Each graph represents the paths of three cells, all in the same cell row at a similar distance from the margin: a reference cell (black), a directly neighboring cell (red) and another more distant cell (blue). Marks in each trace represent the centroid positions of a single cell at 10-minute intervals. Cell paths were tracked along x and y coordinates (the axes perpendicular and parallel to the wound margin, respectively). Traces are representative of three independent experiments. The statistical rates of displacement along the x axis toward the wound area over the period of 0 to 12 hours for each row of cells are indicated at the top of each graph. Rates are mean ± s.e.m. in µm/minute calculated from traces shown and others not shown for a total of n=9 cells for each row position from three separate wounds.
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