spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. Polarised expression of dominant dRTA kAE1 mutants. MDCKI-kAE1(R589H) cells (A), MDCKI-kAE1(S613F) cells (B), MDCKI-kAE1(R901Stop) cells (C,E) and MDCK1-kAE1 cells (D) were polarised on filters. (A-C) Cells were stained for AE1 using Bric 170 (green), (A-C, bottom panels) tight junctions were localised with anti-ZO-1 antibody (red). (D,E) FITC-Bric 6 antibody was added to apical and basolateral surfaces of the intact cell monolayer. (Top panels) Focal planes taken parallel to the epithelium near the centre of the cells (A,B,D) and at the apical surface (C,E). (Bottom panels) Focal planes perpendicular to the epithelium. (A-C, top) Staining with anti-AE1 Bric 170; (A-C, bottom) merged images of anti-AE1 (green) and anti-ZO-1 staining (red). Proteins kAE1(R589H) and kAE1(S613F) are both distributed throughout the polarised cells (A,B, respectively), whereas kAE1(R901Stop) is at the apical membrane (C) and was detected exclusively at the apical surface by FITC-Bric 6 (E). kAE1 was detected exclusively at the basolateral surface by FITC-Bric 6 on filters (D). Scale bar in C, 12 µm (for A-C); scale bar in E, 15 µm (for D,E); AP, apical; BL, basolateral.





Right arrow Return to article