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Fig. 9. S. typhimurium SPI-2 mutant exhibits reduced ability to activate IL-8 expression when colonizing the physiologically relevant apical surface of model intestinal epithelia. The indicated bacterial strains were applied to polarized model epithelia and IL-8 secretion measured as described. (A) Bacteria were applied directly to the basolateral reservoir. (B) Bacteria were applied to the apical reservoir; i.e. the physiological site of colonization. IL-8 in the basolateral compartment was assayed 5 hours later by ELISA. Data are the means±s.e.m. of three parallel experiments.





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